Biol 111: Cellular Metabolism
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Kat Alvarado
DATE \@ "MMMM d, y" September 17, 2015
Biology 111-510
Diffusion Across a Plasma Membrane
An experiment was set up in order to test some of the factors that play a role in the diffusion across a selectively permeable membrane. A membrane that is selectively permeable means that it allows some, but not all substances to pass. The experiment was able to show the experimenters how this lab applies to daily and bodily functions.
In order to set up the experiment, the experimenters obtained 1 small beaker, 2 large beakers, 2 pieces of dialysis tubing and clamps, Lugol’s solution, a bottle of starch, a bottle of a-amylase, a stirring rod, pipets, and 2 glucose test strips. First, a pipet was used to place 4 full pipettes of starch into the small beaker. Then, another pipet was used to place 4 full pipettes of a-amylase into the small beaker with the starch. The experimenters mixed the starch and a-amylase solution with a stirring rod. Then, one of the dialysis bags was opened and filled with 4 pipettes full of the starch/a-amylase solution. The bag was tied off and put into the large beaker 2/3 full with water. Next, 4 pipettes full of the Lugol’s solution was added to the large beaker. It was stirred until the water tuned an orangey color. The experimenters also set up a control bag that contained starch in the dialysis tubing. They placed this bag in the other large beaker that was 2/3 of the way full with water. Lugol’s solution was also added to this beaker and stirred until it turned the same orangey color. The experimenters let the bags sit for an hour before recording their findings.
The initial hypothesis of this experiment was that the beaker with the dialysis tubing containing amylase would test positive for glucose and the bag would not change colors. The null hypothesis was that the beaker with the dialysis tubing containing amylase would not test positive for glucose and there would be a color change in the bag. The initial hypothesis...
DATE \@ "MMMM d, y" September 17, 2015
Biology 111-510
Diffusion Across a Plasma Membrane
An experiment was set up in order to test some of the factors that play a role in the diffusion across a selectively permeable membrane. A membrane that is selectively permeable means that it allows some, but not all substances to pass. The experiment was able to show the experimenters how this lab applies to daily and bodily functions.
In order to set up the experiment, the experimenters obtained 1 small beaker, 2 large beakers, 2 pieces of dialysis tubing and clamps, Lugol’s solution, a bottle of starch, a bottle of a-amylase, a stirring rod, pipets, and 2 glucose test strips. First, a pipet was used to place 4 full pipettes of starch into the small beaker. Then, another pipet was used to place 4 full pipettes of a-amylase into the small beaker with the starch. The experimenters mixed the starch and a-amylase solution with a stirring rod. Then, one of the dialysis bags was opened and filled with 4 pipettes full of the starch/a-amylase solution. The bag was tied off and put into the large beaker 2/3 full with water. Next, 4 pipettes full of the Lugol’s solution was added to the large beaker. It was stirred until the water tuned an orangey color. The experimenters also set up a control bag that contained starch in the dialysis tubing. They placed this bag in the other large beaker that was 2/3 of the way full with water. Lugol’s solution was also added to this beaker and stirred until it turned the same orangey color. The experimenters let the bags sit for an hour before recording their findings.
The initial hypothesis of this experiment was that the beaker with the dialysis tubing containing amylase would test positive for glucose and the bag would not change colors. The null hypothesis was that the beaker with the dialysis tubing containing amylase would not test positive for glucose and there would be a color change in the bag. The initial hypothesis...