Mealworm
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- Date Submitted: 12/13/2013 02:54 AM
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Mayowa Adesina
Biol 131(25)
10/18/13
CHANGE IN ORAL BACTERIAL GROWTH BEFORE AND AFTER MOUTHWASH USE IN A CLASS OF COMMUNITY COLLEGE STUDENTS
INTRODUCTION
One of the major questions regarding oral hygiene is: what helps control oral bacteria? After carrying out experiments to test for the microbiological efficacies of five different mouthwashes on oral bacteria, it was decided to look more in depth into the effect mouthwashes have on oral flora. It is difficult to decide which product is suitable for a particular purpose because of the variations of the antimicrobial efficacy and the mechanical action involved in using the solutions (Pitten, 1995). However, the use of mouthwash will reduce the bacterial population in the oral cavity compared to the amount of bacteria present prior to mouthwash use.
METHODS
A randomized trial was conducted in a class of 54 students divided into 5 groups. The students were instructed to rinse with 30ml of one out of the five mouthwashes provided - Crest, Listerine, Scope, Sweet Talk and Hydrogen Peroxide for 20 to 30 seconds. Two test tubes with broth labeled “before” (before mouthwash use) and “after” (after mouthwash use) were used to culture microbes. A sterile swab was used to collect the oral contents introduced into the broth. After incubating the “before” and “after” broth solutions, each tube was analyzed by assessing the level of turbidity before shaking, then by percent transmittance of light (%T) using a photo spectrometer (Spec 20). The change in the percentage of light transmitted gives more information about bacterial growth or inhibition after using mouthwash.
Following the incubation of the “before” and “after” broth cultures, a disk sensitivity test was carried out to support the data collected from the broth culture analysis. A sample from the “before” broth culture was...
Biol 131(25)
10/18/13
CHANGE IN ORAL BACTERIAL GROWTH BEFORE AND AFTER MOUTHWASH USE IN A CLASS OF COMMUNITY COLLEGE STUDENTS
INTRODUCTION
One of the major questions regarding oral hygiene is: what helps control oral bacteria? After carrying out experiments to test for the microbiological efficacies of five different mouthwashes on oral bacteria, it was decided to look more in depth into the effect mouthwashes have on oral flora. It is difficult to decide which product is suitable for a particular purpose because of the variations of the antimicrobial efficacy and the mechanical action involved in using the solutions (Pitten, 1995). However, the use of mouthwash will reduce the bacterial population in the oral cavity compared to the amount of bacteria present prior to mouthwash use.
METHODS
A randomized trial was conducted in a class of 54 students divided into 5 groups. The students were instructed to rinse with 30ml of one out of the five mouthwashes provided - Crest, Listerine, Scope, Sweet Talk and Hydrogen Peroxide for 20 to 30 seconds. Two test tubes with broth labeled “before” (before mouthwash use) and “after” (after mouthwash use) were used to culture microbes. A sterile swab was used to collect the oral contents introduced into the broth. After incubating the “before” and “after” broth solutions, each tube was analyzed by assessing the level of turbidity before shaking, then by percent transmittance of light (%T) using a photo spectrometer (Spec 20). The change in the percentage of light transmitted gives more information about bacterial growth or inhibition after using mouthwash.
Following the incubation of the “before” and “after” broth cultures, a disk sensitivity test was carried out to support the data collected from the broth culture analysis. A sample from the “before” broth culture was...